Dynamics of Notch signaling in a two-cell assay. Notch reporter (green) is activated upon induction of a Dll1-mCherry in the neighboring cell (CHO cells). Experiment performed in a micropatterned device.
Dynamics of Notch-Delta interactions. Cells expressing Notch-citrine (green) co-cultured with cells expressing Dll1-mCherry (red). Notch extracellular domain trans-endocytose into Dll1 expressing cells upon the induction of Dll1-mCherry (yellow vesicles)
FRAP analysis of Dll1
Membrane dynamics of Dll1 revealed by FRAP- TIRF. A TIRF movie of a Dll1-mCherry cell. By analyzing recovery of bleached area we can analyze diffusion and endocytosis rates.
Planar Cell Polarity
Dynamics of PCP proteins in a two-cell assay. Accumulation of Fat4-citrine (green) and Ds1-mCherry (red) at the boundary between two cells. Experiment performed in a micropatterned device.
The inner ear
Inner ear dynamics 1
Dynamics of hair cells in mouse inner ear explants. Dynamics of hair cell reorganization as observed by 4D movie of an inner ear explant. Atoh1-GFP is marked in the hair cells (P0).
The inner ear
Inner ear dynamics 2
Dynamics of the organ of Corti development in mouse inner ear explants. Dynamics of cellular reorganization as observed by a movie of an inner ear explant (single slice). Atoh1-GFP is marked in red, membrane marker (Tdtomato) in green.